TABLE 1.
PCR primers used in this study
| Primer name | Sequencea | Restriction enzyme |
|---|---|---|
| IspDFor | CATATGAGGGAAGCGGGCGAAGTAG | NdeI |
| IspDRev | CTCGAGTCACCCGCGGAGTATAGCTTG | XhoI |
| IspDNFor | AAGCTTAGCATCCCGCATGCGGGCAGT | HindIII |
| IspDNRev | GGATCCGAGAGTCTGCCCGTCGAGCTG | BamHI |
| IspDCFor | GGATCCACCACCAAACTGGATCTGTTGC | BamHI |
| IspDCRev | GGTACCGGCGATTTCGTTCTCATGATCCG | KpnI |
| IspDint1 | CTCCGACCTTGAAAATCATCT | |
| IspDint2 | TACGACGTCGCGTTTATCC | |
| IspD/FCompPro | TTAATTAAGGCCGGCTGTTAGCATGGAGTAACG | PacI |
| IspD/FCompRev | TTAATTAAGCCAGCTTACCTGCCCAATTGCTG | PacI |
| IspD/FCompSh | TTAATTAAGACGCCAAAGCCGAGACCATCCTT | PacI |
| IspD US Probe F | GACGAGAATCAATGAGACCT | |
| IspD US Probe Rev | AGTGATATCGGCTCGGTGAC |
a
Restriction sites are underlined.