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Calculated kinetic parameters for M. tuberculosis IspDa
| Substrate | Km (μM) | Vmax (pmol/min) | kcat (min−1) | kcat/Km (mM−1 min−1) |
|---|---|---|---|---|
| MEP | 58.5 ± 5.4 | 28.0 ± 0.73 | 0.72 | 12.3 |
| CTP | 53.2 ± 4.5 | 38.6 ± 0.87 | 1.0 | 18.8 |
Each reaction mix contained 38.5 pmol of M. tuberculosis IspD. The Km and Vmax values were calculated from averaged data from three independent experiments, using nonlinear regression analysis (GraFit 5.0.13).
