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. 2007 Oct 19;189(24):8953–8960. doi: 10.1128/JB.01252-07

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Relevant characteristic(s)a Source or reference
Strains
    E. coli TOP10 FmcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 λ Invitrogen
    E. coli BW25113 laclqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 Datsenko and Wanner (8)
        JW2415 E. coli BW25113 ΔcysA Baba et al. (3)
        JW2720 E. coli BW25113 ΔcysC Baba et al. (3)
        JW2722 E. coli BW25113 ΔcysD Baba et al. (3)
        JW3582 E. coli BW25113 ΔcysE Baba et al. (3)
        JW3331 E. coli BW25113 ΔcysG Baba et al. (3)
        JW2732 E. coli BW25113 ΔcysH Baba et al. (3)
        JW2407 E. coli BW25113 ΔcysK Baba et al. (3)
        JW2414 E. coli BW25113 ΔcysM Baba et al. (3)
        JW2418 E. coli BW25113 ΔcysP Baba et al. (3)
        JW2416 E. coli BW25113 ΔcysW Baba et al. (3)
        JW3888 E. coli BW25113 Δsbp Baba et al. (3)
        JW2514 E. coli BW25113 ΔiscS Baba et al. (3)
        JW2515 E. coli BW25113 ΔiscR Baba et al. (3)
    E. coli AB734 Flac-6(del) Shapiro and Baneyx (30)
    E. coli ADA110 AB734 λφibp::lacZ Shapiro and Baneyx (30)
    E. coli ADA310 AB734 λφcspA::lacZ Shapiro and Baneyx (30)
    E. coli ADA410 AB734 λφP3rpoH::lacZ Shapiro and Baneyx (30)
    E. coli ADA510 AB734 λφsulA::lacZ Shapiro and Baneyx (30)
    G. stearothermophilus V Wild-type Telr C. Vásquez (39)
Plasmids
    pBR322 Cloning vector, Apr Tetr Bolivar et al. (4)
    pBluescript-SK Cloning vector, Apr Stratagene
    pBRcobA G. stearothermophilus cobA gene cloned in pBR322, Apr This study
    pBRcysK G. stearothermophilus cysK gene cloned in pBR322, Apr This study
    pBRiscS G. stearothermophilus iscS gene cloned in pBR322, Apr This study
    pSKcobA G. stearothermophilus cobA gene cloned in pBluescript-SK, Apr This study
    pSKcysK G. stearothermophilus cysK gene cloned in pBluescript-SK, Apr This study
    pSKiscS G. stearothermophilus iscS gene cloned in pBluescript-SK, Apr This study
a

Telr, tellurite resistance; Tetr, tetracycline resistance; Apr, ampicillin resistance.