TABLE 1.
Strain or plasmid | Relevant characteristic(s)a | Source or reference |
---|---|---|
Strains | ||
E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR nupG recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 λ− | Invitrogen |
E. coli BW25113 | laclqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | Datsenko and Wanner (8) |
JW2415 | E. coli BW25113 ΔcysA | Baba et al. (3) |
JW2720 | E. coli BW25113 ΔcysC | Baba et al. (3) |
JW2722 | E. coli BW25113 ΔcysD | Baba et al. (3) |
JW3582 | E. coli BW25113 ΔcysE | Baba et al. (3) |
JW3331 | E. coli BW25113 ΔcysG | Baba et al. (3) |
JW2732 | E. coli BW25113 ΔcysH | Baba et al. (3) |
JW2407 | E. coli BW25113 ΔcysK | Baba et al. (3) |
JW2414 | E. coli BW25113 ΔcysM | Baba et al. (3) |
JW2418 | E. coli BW25113 ΔcysP | Baba et al. (3) |
JW2416 | E. coli BW25113 ΔcysW | Baba et al. (3) |
JW3888 | E. coli BW25113 Δsbp | Baba et al. (3) |
JW2514 | E. coli BW25113 ΔiscS | Baba et al. (3) |
JW2515 | E. coli BW25113 ΔiscR | Baba et al. (3) |
E. coli AB734 | F−lac-6(del) | Shapiro and Baneyx (30) |
E. coli ADA110 | AB734 λφibp::lacZ | Shapiro and Baneyx (30) |
E. coli ADA310 | AB734 λφcspA::lacZ | Shapiro and Baneyx (30) |
E. coli ADA410 | AB734 λφP3rpoH::lacZ | Shapiro and Baneyx (30) |
E. coli ADA510 | AB734 λφsulA::lacZ | Shapiro and Baneyx (30) |
G. stearothermophilus V | Wild-type Telr | C. Vásquez (39) |
Plasmids | ||
pBR322 | Cloning vector, Apr Tetr | Bolivar et al. (4) |
pBluescript-SK | Cloning vector, Apr | Stratagene |
pBRcobA | G. stearothermophilus cobA gene cloned in pBR322, Apr | This study |
pBRcysK | G. stearothermophilus cysK gene cloned in pBR322, Apr | This study |
pBRiscS | G. stearothermophilus iscS gene cloned in pBR322, Apr | This study |
pSKcobA | G. stearothermophilus cobA gene cloned in pBluescript-SK, Apr | This study |
pSKcysK | G. stearothermophilus cysK gene cloned in pBluescript-SK, Apr | This study |
pSKiscS | G. stearothermophilus iscS gene cloned in pBluescript-SK, Apr | This study |
Telr, tellurite resistance; Tetr, tetracycline resistance; Apr, ampicillin resistance.