TABLE 2.

Primers used in PCR and quantitative RT-PCR

Primer name	Sequence	PCR product
For G. stearothermophilus V:
cobAPH	5′-AAGCTTTTCGCTCCGGATGTTGCCGATTAT-3′	cobA gene + promoter
cobAH3	5′-AAGCTTCGGTCCCGGCGATGAAAACGTGATTACCGT-3′	cobA gene + promoter
cysKPH	5′-AAGCTTCGCTTACAGACTATTCCGCCTGTCT-3′	cysK gene + promoter
cysKH3	5′-AAGCTTGTCTTCGAATTGGTAAAGCGGCGTGCTTAAGTAGC-3′	cysK gene + promoter
iscSPH	5′-AAGCTTTCGCAGACATTACTAGAACGGTTGCTGTAG-3′	iscS gene + promoter
iscSH3	5′-AAGCTTATGAATCTTGAACAAATAAGAAAAGATAAC-3′	iscS gene + promoter
gapA3	5′-GGTGATGTGTTTACGAGCAG-3	gapA probe
gapA5	5′-GTAAAGTTGGTATTAACGGTTTTGG-3′	gapA probe
scobA5	5′-GTACCTCTGCTTCTTCCCCG-3′	cobA probe
subiE5	5′-CTGCACAAAGCGAATCCGTT-3′	ubiE probe
scysK3	5′-ACTCATCGTATCTGGCATGA-3′	cysK probe
siscS3	5′-CGAATACACGGTAAAACAAT-3′	iscS probe
For E. coli:
sgapA3	5′-GGTGATGTGTTTACGAGCAG-3′	gapA probe
sgapA5	5′-GTAAAGTTGGTATTAACGGTTTTGG-3′	gapA probe
scysA3	5′-AACCAGCGGGCATATTCGCTTCCACGGCAC-3′	cysA probe
scysA5	5′-CAAGCAGCAGAATTTGCGGTTCCACAGCCA-3′	cysA probe
scysB3	5′-CTGCGCGATTAAAGGCAGTATCCAGTTCTG-3′	cysB probe
scysB5	5′-ACCTGGCCGGATAAAGGTTCACTGTATATCGC-3′	cysB probe
scysC3	5′-TTTAGCGATGCCGATCGTAAAGAGAATATC-3′	cysC probe
scysC5	5′-GTTCACCATTGAGATGAATTTCTGCCGATT-3′	cysC probe
scysE3	5′-TCTGTGACGTTCCAGGTCGATATTCACCCG-3′	cysE probe
scysE5	5′-GCTGGTCCATATCCATTGATGGCTTATCGCTG-3′	cysE probe
scysG3	5′-ACCACCACATCTGCCTGCTGAATTTGTTGC-3′	cysG probe
scysG5	5′-ACTTGAATCACTGCTGCCGTTACATCTGGG-3′	cysG probe
scysI3	5′-GTGAAAACTCGATGGCTTGCGTGTCATTCC-3′	cysI probe
scysI5	5′-CCCTATCAGTTCATCAAGCGACGCCAGGAT-3′	cysI probe
scysK3	5′-CTTTCAGCAGCTTGCGGCGTTCAATACTCA-3′	cysK probe
scysK5	5′-TGAAGATAACTCGCTGACTATCGGTCACACGC-3′	cysK probe
scysM3	5′-GGCGAAGGAAAGCTGCTCGATCAGTTCAAT-3′	cysM probe
scysM5	5′-GATGAATATCCAGCACCTCATCCACCAGAGAAGC-3′	cysM probe
scbl3	5′-GCCCGCAAAGGTTTGCTGGCAGATATTGTA-3′	cbl probe
scbl5	5′-TTTCCTCTTCACTGCTTTCCATCACCTGCC-3′	cbl probe