FIG. 3.
Flow cytometric profiles of DK1622 and the DnaBA116V mutant grown at 28 and 37°C: flow cytometric profiles of DK1622 incubated at 28°C (A), the DnaBA116V mutant incubated at 28°C prior to a shift to 37°C (B), and the DnaBA116V mutant after 3 h (C), 6 h (D), 9 h (E), and 12 h (F) of incubation at 37°C. Cells were grown in CTTYE medium and fixed in paraformaldehyde as described in Materials and Methods prior to flow cytometric analysis. The y axis represents the number of cells counted for each fluorescence unit, and the x axis represents arbitrary fluorescence units ranging from 0 to 1,000 on a linear scale.