FIG. 3.

EMSAs showing that ORF156 binds to ORF157-iteron complexes. Thirty femtomoles of the 48-bp γ-³²P-labeled pBtoxis iteron probe was added to each EMSA reaction mixture. Lane 1, negative control without protein; lane 2, 360 pmol purified rORF156 added to the reaction mixture; lanes 3 to 8, 30 pmol purified rORF157 added to reaction mixtures with different amounts of purified rORF156 (0, 360, 240, 120, 60, and 30 pmol). The locations of DNA-protein complexes (arrows) and unbound labeled iterons (asterisk) are shown.