TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| B. thuringiensis | ||
| 4Q7 | Acrystalliferous strain of B. thuringiensis subsp. israelensis | Bacillus Genetic Stock Center |
| 4Q5 | Plasmid-cured mutant of B. thuringiensis subsp. israelensis; bears only pBtoxis plasmid | Bacillus Genetic Stock Center |
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | |
| Top10 | Invitrogen | |
| Plasmids | ||
| pC194 | Plasmid from Staphylococcus aureus containing a chloramphenicol resistance gene | 9 |
| pUC19 | High-copy-no. E. coli vector containing MCS; Ampr | 23 |
| pUCCl | pUC19 containing the chloramphenicol resistance gene from pC194 | This work |
| pUCSw | pUCCl containing 2.2-kb mini-replicon of pBtoxis | This work |
| pUCp | pUCCl containing 191-bp fragment upstream of ORF157 | This work |
| pUCID | pUCCl containing 97-bp fragment (48-bp iterons, 37-bp spacer, plus putative dnaA box) | This work |
| pUCIDM | pUCCl containing iterons, 37-bp spacer, and mutated dnaA box | This work |
| pUCIS | pUCCl containing iterons and 37-bp spacer | This work |
| pUCSD | pUCCl containing 37-bp spacer and putative dnaA box | This work |
| pUCI | pUCCl containing 48-bp iteron sequence | This work |
| pBtKp | pUCE containing 6.3-kb KpnI fragment of pBtoxis | 18 |
| pBAD/myc-HisB | Vector for expression of recombinant proteins containing C-terminal 6-His tag in E. coli | Invitrogen |
| pBAD156 | pBAD/myc-HisB containing pBt156 gene | This work |
| pBAD157 | pBAD/myc-HisB containing pBt157 gene | This work |