TABLE 2.
Primers used for PCR
| Primer | Sequence (5′ to 3′)a |
|---|---|
| pIDna-F | CCCAAGCTTTAAAGGTTTAAATAACAGTTTAAA |
| pIDna-R | AACTGCAGGAGAGTGGAAAAATTCACCTAACC |
| pP-F | CCCAAGCTTAAATTTAATTTTAGGTTTAAATTT |
| pP-R | AACTGCAGAACTCCCATCTGTTTAATTAATTC |
| pIDM-F | AAAAGTACTCACCAGTCACAGAAAAGCATCTT |
| pIDM-R | CGGAATTCGTCTTGGGGATTCACCTAACCTATTTATATCA |
| pIS-R | CGGAATTCATTCACCTAACCTATTTATATCAC |
| pSD-F | CCCAAGCTTTACCATTCCCAGTGTGATATAAAT |
| pSD-R | CATGCCATGGAATAATAGAAAGAGAAAAAGCATT |
| pBAD156-F | CATGCCATGGCAATGTTATTAAACAGTAATGAACTA |
| pBAD156-R | GCTCTAGACCACGTTTTTTAAATGGATTTGA |
| pBAD157-F | CATGCCATGGCAATGAATAGGGATCACTTTTATACG |
| pBAD157-R | GCTCTAGACCTAAATTACGCATTTCCATTGC |
a
Restriction endonuclease cleavage sites are typed in boldface, and the mutated nucleotide bases are underlined.