FIG. 6.

MDP1 mediates cessation of glycolipid biosynthesis at the stationary growth phase. (A) Lipid synthesis was measured by addition of [¹⁴C]acetate to a bacterial culture. The TMM and MAMEs from 10⁸ bacteria, including the M. smegmatis parent strain (wild type [WT]), the MDP1 KO strain (KO), and the complemented strain (Comp), were extracted on days 3 and 7 and fractionated by TLC. The radioactivities of synthesized TMM and MAMEs were visualized by using the BAS system. (B to D) Radioactivities of TMM (B), alpha-MAME (C), and alpha′-MAME (D) extracted from a bacterial culture after 7 days were quantified by using the BAS system software and compared for the wild-type, MDP1 KO, and complemented strains. The values are means ± standard deviations of five experiments. PSL, photo-stimulated luminescence.