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. 2007 Sep 14;189(22):8377–8380. doi: 10.1128/JB.01199-07

FIG. 2.

FIG. 2.

Effects of Rrp2, RpoN, and RpoS on BBK32 protein expression in B. burgdorferi. Wild-type and various mutant strains of B. burgdorferi were cultivated at 37°C either in BSK-H medium at pH 7.5 (A and C) or in BSK-H medium adjusted to pH 6.8 (B). Spirochetes were harvested at late logarithmic phase (∼5 × 107 spirochetes/ml) and subjected to immunoblotting. Each gel lane was loaded with approximately 5 × 107 spirochetes. wt, wild-type B. burgdorferi strain BbAH130; rpoN−, RpoN-deficient mutant; rpoN−/+, RpoN-deficient mutant complemented with a wild-type copy of rpoN; rpoS−, RpoS-deficient mutant; rpoS−/+, RpoS-deficient mutant complemented with a wild-type copy of rpoS; rpoN− + flgBp-rpoS, RpoN-deficient mutant complemented with a constitutively expressed wild-type copy of rpoS; rrp2(G239C), rrp2 mutant; rrp2+, a wild-type rrp2 allele was restored in the rrp2 mutant. Monoclonal antibodies used for the immunoblots are indicated on the left.