FIG. 5.

DivL promotes DivK phosphorylation. (A) In vivo phosphorylation was measured by immunoprecipitation of CtrA and DivK from cells grown at 28°C and labeled for 4 min with [γ-³²P]ATP. CtrA (B) and DivK (C) protein levels were determined by Western blotting. For panels A to C, anti-CtrA and anti-DivK sera were used to recognize CtrA and DivK, respectively. Lane 1, KR684 (CB15N); lane 2, KR635 (divL510). (D) Ratios of phosphorylated to total protein were calculated for three independent experiments with KR684 and KR635 and were normalized to the wild-type strain. Error bars represent standard deviations.