TABLE 2.
Rapid attachment assays with P. aeruginosa strains PAO1, WFPA800 (Δpsl), WFPA818 (ΔpslH), and WFPA819 (ΔpslI) and complemented strainsa
| Strain | Crystal violet absorbance (A540)b |
|---|---|
| PAO1 (wild type) | 0.208 ± 0.047 |
| WFPA800 (Δpsl) | 0.058 ± 0.015 |
| WFPA818 (ΔpslH) | 0.049 ± 0.005 |
| WFPA819 (ΔpslI) | 0.021 ± 0.004 |
| WFPA818/pMA10 | 0.158 ± 0.014 |
| WFPA819/pMA11 | 0.187 ± 0.044 |
a
The strains were assayed after 30 min of static incubation at room temperature in a microtiter dish (16). The deletion of pslH or pslI gave a psl null phenotype in this assay.
b
Data are means ± standard deviations for three replicates.