TABLE 2.

Fatty acid compositions of total membrane lipid extracts from S. pneumoniae strains^a

Fatty acid	% in S. pneumoniae strains
	708	SH9b	SH11
n-C12:0	0.16 ± 0.07	2.31 ± 0.68	0.62 ± 0.06
n-C14:1	0.92 ± 0.02	0	0
n-C14:0	1.29 ± 0.30	4.43 ± 1.12	5.51 ± 0.51
n-C16:1Δ7	4.29 ± 0.51	0	3.88 ± 0.10
n-C16:1Δ9	15.69 ± 1.39	tr	13.98 ± 0.45
n-C16:1Δ11	8.21 ± 0.26	0	3.68 ± 0.24
n-C16:0	7.50 ± 1.65	9.25 ± 1.76	27.68 ± 2.45
n-C18:1Δ9	3.4 ± 0.73	83.29 ± 1.72	4.16 ± 0.28
n-C18:1Δ11	50.3 ± 0.71	0	33.92 ± 2.31
n-C18:1Δ13	6.53 ± 0.90	0	2.23 ± 0.16
n-C18:0	1.67 ± 0.32	0.71 ± 0.21	4.1 ± 0.41
All UFAs	89.34 ± 4.52	83.69 ± 1.74	61.85 ± 3.54

^aCells were grown in AGCHYE medium to exponential phase at 37°C. The total lipids were extracted and transesterified to obtain fatty acid methyl esters, and products were identified by gas chromatography-mass spectrometry. The values are the means ± standard deviations of three independent experiments and are percentages of the total fatty acids.

^bCultures of SH9 were supplemented with 0.1 mM oleate.