TABLE 3.
Fatty acid compositions of total membrane lipid extracts from S. mutans strainsa
| Fatty acid | % in S. mutans strains
|
||
|---|---|---|---|
| SMU159 | SA20b | SA21 | |
| n-C12:0 | 0.64 ± 0.14 | 5.94 ± 0.17 | 3.23 ± 0.07 |
| n-C14:1 | 0 | 0 | 0.6 ± 0.07 |
| n-C14:0 | 4.2 ± 1.10 | 4.28 ± 0.80 | 10.53 ± 0.84 |
| n-C16:1Δ7 | 2.07 ± 0.27 | 0 | 2.21 ± 0.27 |
| n-C16:1Δ9 | 0.91 ± 0.27 | tr | 1.75 ± 0.03 |
| n-C16:1Δ11 | 1.09 ± 0.10 | 0 | 1.12 ± 0.23 |
| n-C16:0 | 37.4 ± 2.2 | 43.08 ± 2.20 | 46.68 ± 3.2 |
| n-C18:1Δ9 | 3.69 ± 0.41 | 18.86 ± 0.35 | 2.08 ± 0.35 |
| n-C18:1Δ11 | 20.75 ± 0.61 | 0 | 7.28 ± 0.18 |
| n-C18:1Δ13 | 0.81 | 0 | 0 |
| n-C18:0 | 13.34 ± 1.7 | 10.49 ± 1.12 | 19.36 ± 1.12 |
| n-C20:1 | 13.2 ± 0.7 | 13.96 ± 1.73 | 2.81 ± 0.23 |
| n-C20:0 | 1.85 ± 0.3 | 2.85 ± 0.30 | 2.12 ± 0.27 |
| All UFAs | 42.52 ± 2.46 | 32.82 ± 2.08 | 17.25 ± 1.29 |
a
Cells were grown in AGCHYE medium to exponential phase at 37°C. The total lipids were extracted and transesterified to obtain fatty acid methyl esters, and products were identified by gas chromatography-mass spectrometry. The values are the means ± standard deviations of three independent experiments and are percentages of the total fatty acids.
b
Cultures of SA20 were supplemented with 0.100 mM oleate.