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. 2007 Sep 14;189(22):7998–8004. doi: 10.1128/JB.01073-07

FIG. 4.

FIG. 4.

Activity of wild-type SltF and mutant proteins. Agar plates containing M. luteus as a substrate were used to test the activity of the pure proteins SltF (spot 1), SltF E57A (spot 2), SltF E83A (spot 3), egg white lysozyme (EWL) (spot 4). The clear zone is due to enzymatic activity degrading peptidoglycan from “M. lysodeikticus.” Pure protein (7 μg) was deposited, and the lisoplate was incubated 12 h at 37°C.