Relative transcription of the dps, pflB, and osmB genes in MG1655 (WT) and EB1.3 (isogenic rpoS mutant), as determined by real-time PCR. Bars: □, strains transformed with pT7-7; ▪, strains transformed with pT7CsgD. Relative transcription values were set to 1 for both MG1655/pT7-7 and EB1.3/pT7-7 for better comparison of the CsgD-dependent effects in either strain.