TABLE 2.
Gene characteristics
| Band no.a | Gene productb | Predicted molecular mass (Da)c | Gene function (reference) | Regulation of corresponding gene (reference) |
|---|---|---|---|---|
| 1 | PflB* | 85,357 | Pyruvate formate lyase I (56) | Induced by ArcA and FNR (61) |
| 2 | GadA* | 52,685 | Glutamate decarboxylase (64) | Positively regulated by rpoS, CRP, gadE, gadW, and gadX; negatively regulated by H-NS (21, 66) |
| 3 | TnaA† | 52,773 | Tryptophan deaminase (tryptophanase) (22) | Positively activated by rpoS and by CRP (33, 74) |
| 3 | GatZ† | 47,109 | Subunit of tagatose-1,6-bisphosphate aldolase 2 (42) | Positively activated by CRP (74); repressed by ArcA (60) |
| 4 | GatY† | 30,812 | Subunit of tagatose-1,6-bisphosphate aldolase 2 (42) | Same transcription unit as gatZ |
| 5 | WrbA* | 20,846 | Quinone reductase; response to oxidative stress (45) | Positively regulated by rpoS (33, 46, 71) |
| 6, 7, and 11 | Dps* | 18,695 | Bacterial ferritin, DNA protection during stationary phase (39, 73) | Positively regulated by rpoS (2, 33, 46, 71) |
| 8 | CsgD* | 24,935 | Transcriptional regulator (28) | Positively regulated by rpoS, hns, and ompR (25, 58) |
| 9 | CsgG* | 30,557 | Outer membrane protein; curli transport component (38) | Same transcription unit as csgD |
| 10 | OmpW* | 22,928 | Outer membrane protein; receptor for colicin S4 (49) | Positively regulated by FNR (18) |
a
As in Fig. 2. Band 3 was identified as a mixture of two proteins and thus appears twice, once for each protein.
b
*, Increased expression in MG1655/pT7CsgD; †, decreased expression in MG1655/pT7CsgD.
c
Predicted molecular masses were obtained from the EcoCyc database (http://www.ecocyc.org/).