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. 2007 Sep 14;189(22):8024–8033. doi: 10.1128/JB.01047-07

FIG. 4.

FIG. 4.

(A) φ29 protein p6 binding to the φ29 right terminus (φ6) and plasmid region P1. B. subtilis 110NA cells producing φ29 protein p6 were grown and treated with 34 μg of chloramphenicol/ml together with or without 500 μg of novobiocin/ml. Cells were infected with φ29 sus14(1242) and cross-linked 40 min later. IC values for each DNA region in the absence or presence of novobiocin are shown in the left panel. The binding increase in the presence of novobiocin, expressed as the IC+Nov/IC−Nov ratio, is shown in the right panel. (B) GA-1 protein p6 binding to the φ29 right terminus (φ6) and plasmid region P1. GA-1 protein p6 synthesis was induced in B. subtilis 110NA cells as described in Materials and Methods. Cells processed as before were infected with φ29 sus14(1242) and cross-linked 40 min later. IC values in the absence or presence of novobiocin are shown in the left panel. The binding increase in the presence of novobiocin, expressed as the IC+Nov/IC−Nov ratio, is shown in the right panel.