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. 2007 Sep 21;189(22):8130–8138. doi: 10.1128/JB.00820-07

FIG. 4.

FIG. 4.

Inhibition of proteolysis of CII from HflB by CIIIC. (A) The top part of the panel shows the CII band on a 17.5% SDS-PAGE, and the bottom part of the panel represents the amount of CII remaining at various times of digestion, obtained by densitometry of the gel. Lane U, undigested control; lanes 2 to 6, digestion of CII (0.4 nmol) by HflB (0.04 nmol) for the indicated times (5 to 80 min); lanes 7 to 11, digestion of CII in the presence of CIIIC (3 nmol). Each data point is the mean value of three identical experiments. (B) The top part of the panel shows the CII band on a 17.5% SDS-PAGE, and the bottom part of the panel represents the amount of CII (0.4 nmol) remaining after digestion by HflB (0.04 nmol) for 60 min in the presence of different concentrations of CIIIC. Lane U, undigested control; lanes 1 to 5, 0 to 6 nmol of CIIIC.