FIG. 5.

Inhibition of proteolysis of CIII by CIIIC. (A) CIII band on a 17.5% SDS-PAGE. Two nanomoles of CIII, alone (top part of the panel) or in the presence of 3 nmol of CIIIC (middle part of the panel), was digested by HflB (40 pmol). CIII was also digested by HflB preincubated with CIIIC (bottom part of the panel). Lane U, undigested control; lanes 1 to 5, treated with HflB for 5, 10, 20, 40, or 80 min. (B) Amounts of CIII remaining after various times of digestion, obtained by densitometry of the gels. Symbols: ○, CIII alone; ▪, HflB added to the CIII-CIIIC mixture; •, HflB preincubated with CIIIC prior to the addition of CIII.