TABLE 1.
Differential expression of V. fischeri genes in response to 3OC6-HSL
| Gene or intergenic region (gene location)a | Annotation | Fold regulation at ODb660 of:
|
|
|---|---|---|---|
| 0.3 | 1.5 | ||
| VF1161 (1290494-1291648) | Periplasmic component of efflux system | 25.6 | 10.3 |
| VF1162 (1291644-1292921) | Outer membrane efflux protein (TolC) | 12.5 | 7.1 |
| VF1163 (1292921-1294135) | Export ABC transporter permease protein | 8.9 | 6.4 |
| VF1164 (1294142-1295422) | Export ABC transporter permease protein | 7.2 | 5.4 |
| VF1165 (1295418-1296140) | ABC transporter ATP-binding protein | 7.3 | 5.4 |
| VF1246 (1390844-1389936) | Putative omptin family serine protease | 31.8 | 12.2 |
| VF1247 (1391269-1391697) | Peptidoglycan-binding protein, LysM | 2.6 | 2.7 |
| VF1348 (1493989-1492961) | Hypothetical cytosolic protein | 9.5 | 6.7 |
| VF1349 (1495924-1494185) | Alkaline serine protease | 8.9 | 6.6 |
| VF1725 (1948180-1949709) | Secretory tripeptidyl aminopeptidase | 27.3 | 16.0 |
| VF1977* (2215928-2215569) | Protein YgiW precursor | 6.1 | ND |
| VF1978* (2216620-2215988) | Accessory colonization factor A (AcfA)-like protein | 16.1 | ND |
| VF2034 (2270424-2270026) | Hypothetical polypeptide | 2.6 | ND |
| VF2035 (2270891-2270424) | Hypothetical polypeptide | 2.7 | ND |
| VF2036 (2271435-2270905) | Possible phage regulatory protein | 2.7 | ND |
| VFA0090 (99228-98098) | Astacin peptidase | 29.2 | 9.9 |
| VFA0373 (420748-422019) | Mechanosensitive ion channel | 9.7 | 3.5 |
| VFA0622 (702734-702162) | Hypothetical protein | 7.2 | 3.6 |
| VFA0834 (939286-941157) | NirV precursor | ND | 3.2 |
| VFA0894 (1014502-1016295) | Bacterial immunoglobulin-like protein | 35.9 | 16.7 |
| VFA0895 (1016495-1016854) | Permease (major facilitator superfamily) | 3.3 | 2.6 |
| VFA0918* (1044437-1043730) | luxG | 9.0 | 5.9 |
| VFA0919* (1045577-1044450) | luxE | 10.0 | 6.7 |
| VFA0920* (1046636-1045659) | luxB | 8.1 | 4.9 |
| VFA0921* (1047714-1046650) | luxA | 10.6 | 6.0 |
| VFA0922* (1048660-1047740) | luxD | 8.9 | 5.0 |
| VFA0923* (1050115-1048679) | luxC | 14.4 | 7.4 |
| VFA0924* (1050725-1050156) | luxI | 13.9 | 5.7 |
| VFA1058* (1194722-1195164) | qsrP | 37.2 | 10.9 |
| VF1866 to VF1867 (2105494-2106116) | Intergenic region | ND | 3.8 |
| VFA0643 to VFA0644 (719637-720412) | Intergenic region | 3.5 | 2.4 |
| VF1615 (1814208-1814837) | Hypothetical protein | ND | −2.7 |
a
Genes marked with an asterisk have been identified as 3OC6-HSL regulated elsewhere. VF designates genes on the large chromosome, and VFA designates genes on the small chromosome.
b
“Fold regulation” refers to transcript levels in the presence versus absence of 3OC6-HSL. ND, no significant difference. All significant differences show a P value of <0.001.