Figure 4.

Promoter sensitivity to TAF_II250 correlates with sensitivity to Tat-mediated repression. A series of promoters linked to the reporter CAT with known sensitivities to Tat-mediated repression (as indicated) were transfected into tsBN462 cells. Promoter activity was measured at the permissive (32°C) or restrictive (39°C) temperatures. To allow a direct comparison of the different promoters, activity was normalized to the activity of each at 32°C. Promoters sensitive to Tat repression were 313 (class I), b2 m (β₂microglobulin), and pSV3 (basal SV40 promoter); resistant promoters were pSV2 (SV40 enhancer/promoter) and MuLV (murine leukemia virus).