Table 2.
HIV-1 TAT101 inhibits in vitro transcription of the MHC class I, but not the HIV LTR or MuLV LTR, promoter
| Relative promoter
activity in vitro
|
|||
|---|---|---|---|
| MHC Class I | HIV LTR | MuLV | |
| A. | |||
| No additions | 1.0 | 1.0 | |
| GST | 0.84 | 0.92 | |
| GST-Tat101 | 0.41 | 2.5 | |
| B. | |||
| GST | – | 1.0 | |
| GST-SNAP23 | 1.0 | – | |
| GST-Tat101 | 0.32 | 1.05 | |
| GST-Tat67-101 | 0.32 | 1.0 | |
| GST-Tat101K41T | 0.50 | ||
| GST-Tat1-67 | 0.91 | 1.05 | |
In vitro transcription reactions with each of the three promoters—class I, HIV LTR, and MuLV—were performed by using the promoters fused to the CAT gene. Recombinant GST fusion proteins (0.5 μg) were added where indicated. A and B quantitate the results of two independent and representative experiments using the class I promoter. Analysis of the HIV and MuLV LTRs were done in parallel with the class I promoter experiments; each has been repeated twice.