FIG. 1.

C. jejuni ppk1 and generation of a single insert Δppk1 disruption strain. (A) Genomic location of the ppk1 gene is conserved among the sequenced and annotated strains C. jejuni 81-176, C. jejuni 11168 and C. jejuni RM1221. MUMer alignment was performed by using the CampyDB gene viewer (17). Hypothetical proteins are represented by an asterisk (*), and genes with no predicted orthologues are colored gray. (B) The approximate site of the cat-marked insertion-deletion mutation generated in C. jejuni 81-176 ppk1 is shown. The resultant mutant strain was designated Δppk1.