FIG. 2.

Poly-P accumulates in wild-type and ppk1* strains at later growth stages but remains at low levels in Δppk1 and ΔspoT strains. Wild-type (WT) C. jejuni 81-176, Δppk1 mutant, the complemented strain ppk1*, and the ΔspoT mutant were grown microaerobically in shaking broth culture to early log phase and diluted to an OD₆₀₀ of 0.05. Cultures (1 ml) were harvested at 2, 10, and 24 h and assayed for CFU/ml (A) and poly-P levels versus total cellular protein (B). Triplicate samples were harvested and assayed for each time point. Statistical significance (P < 0.05) is represented by an asterisk.