FIG. 4.
Poly-P is required for C. jejuni osmotic stress survival. (A) C. jejuni 81-176 wild-type and Δppk1 strains were grown to mid-log phase and shifted to MH broth with or without 0.25 M NaCl. Growth and/or survivability was monitored by CFU/ml plate counts. (B) Wild-type, Δppk1, and ppk1* strains were grown to mid-log phase in MH broth and serially diluted (1:10) from 2 × 105 to 2 × 101 CFU/ml and spotted onto 0.17 M NaCl MH agar plates. Growth was assessed after 48 h under microaerobic conditions.