FIG. 5.
The Δppk1 mutant exhibits increased static biofilm formation. Microtiter PVC plates were inoculated with C. jejuni in MH broth at OD600s of 0.02 and 0.20. At 48 h, biofilms were qualitatively observed by crystal violet staining. (A) Representative wells from the OD600 0.02 inoculation are shown for wild-type, Δppk1, and ppk1* strains. (B) Biofilm formation was quantified by the addition of 80% DMSO to each crystal violet-stained well, followed by OD570 absorbance measurements of solubilized crystal violet. Statistical significance (P < 0.05) is represented by an asterisk.