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Construction of a Δalr mutant of M. smegmatis mc2155. Replacement of the chromosomal alr gene with the kanamycin cassette (aphA-3 gene) results from crossover events in the right and left flanks. Restriction sites of SmaI and fragments expected in Southern hybridization analysis are indicated for wild-type mc2155 (WT) and for the Δalr mutant (KO). LF, left flank; RF, right flank.
