FIG. 3.
Growth profiles of M. smegmatis mc2155 and mutants. (a) Growth of Δalr strain DM22 in LBT medium as a function of initial d-alanine concentration. (b) Growth of mc2155 (squares), Δalr strain DM22 (circles), and complemented Δalr strain DM30 (triangles) in LBT medium containing 1 mM d-alanine. At the 4-h time point (marked by arrow), cultures were collected by centrifugation, washed with LBT medium, and then split into LBT medium without (open symbols) or with (closed symbols) 1 mM d-alanine.