TABLE 2.
Antibiotic resistance and growth requirements of six candidate alr deletion mutants (DM21 to DM26) selected in the presence of d-alanine
| Colony/strain | Growth on selective mediuma
|
|||
|---|---|---|---|---|
| Kanamycin | Sucrose | Gentamicin | Without d-Ala | |
| DM21 | + | + | + | + |
| DM22 | + | + | − | − |
| DM23 | + | + | − | − |
| DM24 | + | + | − | − |
| DM25 | + | + | − | − |
| DM26 | + | + | + | + |
a
M. smegmatis strain DM5, which harbors the Δalr delivery vector pDM2, was grown to exponential phase at 28°C in LBT medium supplemented with 5 mM d,l-alanine. Aliquots of the culture were plated onto LBT-kanamycin-sucrose plates supplemented with 5 mM d,l-alanine. The plates were incubated at 40°C to select for chromosomal integration of the vector.