FIG. 4.

Mass spectra of the acetylated derivatives of the NpdA2 transformation product identified as 5-chlorohydroxyquinol. Transformations were carried out using cleared lysates of E. coli BL21(DE3)(pLysS, pLP261) as the source of NpdA2, as described in Materials and Methods. The transformation substrates were 2,4,5-trichlorophenol (A) and 4-chlorocatechol (B). Following incubation for 30 min at 25°C, reaction mixtures were acetylated with acetic anhydride and analyzed by GC-MS.