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Purification of hydroxyquinol 1,2-dioxygenase from Arthrobacter sp. strain JS443
| Purification step | Total protein (mg) | Total activity (μmol min−1) | Sp act (μmol min−1 mg protein−1) | Yield (%) |
|---|---|---|---|---|
| Crude lysate | 620 | 12 | 0.019 | |
| Ammonium sulfate | 610 | 18 | 0.029 | |
| Dialysis | 460 | 200 | 0.43 | 100 |
| Q-Sepharose Fast Flow | 6.8 | 95 | 14 | 48 |
| Sephacryl 100 | 0.95 | 30 | 32 | 15 |
| Phenyl Superose | 0.12 | 6.7 | 58 | 3.3 |
