TABLE 1.
Strains, plasmids, and compatibility phenotypes on M. truncatula A17 and A20
| Strain or plasmid | Characteristics or genotype (source)a | Compatibility
|
|
|---|---|---|---|
| A17 | A20 | ||
| S. meliloti strains | |||
| NRG185 | WTb from M. sativa; North America (37) | +c | −d |
| NRG34 | WT from M. sativa; North America (37) | + | − |
| Rm41 | WT from M. sativa; Europe (42) | − | + |
| NRG247 | WT from M. sativa; North America (37) | − | + |
| Rm1021 | Derivative of S. meliloti SU47 (35) | +/−e | +/− |
| GRC01 | NRG185 exoB::Tn5 (EPS−) (this work) | − | − |
| AK631 | Rm41 exoB631 (EPS−) (42) | − | − |
| PP674 | AK631 rkpA::Tn5 (EPS− KPS−) (40) | − | − |
| PP699 | Rm41 rkpA::Tn5 (KPS−) (40) | − | + |
| TOR33 | NRG185 rkpA::Tn5 (KPS−) (this work) | + | − |
| TJR41 | Rm41 pEX312 (this work) | +/− | +/− |
| TJR42 | Rm41 pD56 (this work) | − | + |
| NRG185 + Rm41 | Coinoculation on M. truncatula A17 | + | NTf |
| Plasmids | |||
| pEX312 | Tcr pLAFR1 with Rm1021 exo subclone (33) | ||
| pD56 | Tcr pLAFR1 with Rm1021 exo subclone (33) | ||
| pPP428::Tn5-674 | Tcr pLAFR1 rkp-1 (pPP428 rkpA::Tn5) (42) | ||
| pRK602 | pRK600′ΩTn5 (exoB::Tn5) (31) | ||
a
EPS, exopolysaccharide; KPS, K-antigen polysaccharide.
b
WT recovered from alfalfa nodules.
c
+, compatible (Fix+); numerous (25 to 45) functional nodules and healthy dark green plants.
d
−, incompatible (Fix−); no functional nodules, only pseudonodules, and unhealthy light green to yellow plants.
e
+/−, compatible (Fix+) but low efficiency; few functional nodules and light green plants.
f
NT, not tested.