TABLE 2.
Plasmids used in this study
| Plasmid | Marker(s) | Properties | Reference or source |
|---|---|---|---|
| pCSEL21 | Apr | pIC20R with gene-GFP insertion | 48 |
| pCSV3 | Spr Smr | pRL500 with substituted Apr gene | 49 |
| pCSEL24 | Apr Spr Smr | pBR322 containing Anabaena sp. 2-kb nucA-nuiA fragment and C.S3 cassette | E. Flores, CSIC |
| pGEMT | Apr | A/T cloning vector | Promega Corp. |
| pIM125 | Apr | pGEMT with PCR fragment of alr2887 (2,300-bp insert) | This study |
| pIM136 | Apr Kmr | pIM125 with C.K3 | This study |
| pIM181 | Cmr Emr Kmr | pRL271 with insert of pIM136 | This study |
| pNMP-alr2887-GFP | Spr Smr | pCSV3 with fragment of alr2887 | This study |
| pNME-alr2887-GFP | (Apr) Spr Smr | pCSEL24 with alr2887 promoter-gfp fusion | This study |
| pNMΔ-alr2887 | Spr Smr | pCSV3 with fragment of alr2887 | This study |
| pRL271 | Cmr Emr | Positive selection vector with sacB | 5 |