TABLE 3.
Primers used for cloning
Primer | Oligonucleotide sequence |
---|---|
NME-alr2887-GFP-F | 5′-ATCGATCGATACAGGTACAGGTAAAACCCTGTTA-3′ |
NME-alr2887-GFP-R | 5′-ATCGGATATCATAGAATAAGTGTTGTCCTTTCACCG-3′ |
NMP-alr2887-GFP-F | 5′-ATCGATCGATTTAGCAGGGCTGTGGAACCAAT-3′ |
NMP-alr2887-GFP-R | 5′-ATCGGATATCCTGACTACTAATTAATGCTCTAGAAGT-3′ |
NMΔ-alr2887-F | 5′-ATCGGGATCCCCAGCAGATACTCAGTCACCAA-3′ |
NMΔ-alr2887-R | 5′-ATCGGGATCCAGCAATACGGACTTGTTCATCTGC-3′ |
Oligo 309 | 5′-CTCGAGGGTATCGATAGAGAAC-3′ |
Oligo 310 | 5′-CTCGAGGCCATCATCTCGGTAATTG-3′ |
GFP1 | 5′-CCTCTCCACTGACAGAGAATTTTT-3′ |
GFP2 | 5′-GGGTAAGTTTTCCGTATGTTGCAT-3′ |
alr2887-P1 | 5′-AACCTGATTCCCAATGCCAATCC-3′ |
alr2887-P4 | 5′-AAATCTCCTTCGCAGTCCTCAATTAA-3′ |