FIG. 7.

Incorporation of EA-P into a cobamide known as norcobalamin. HPLC was performed with corrinoid extracts from strain JE8836 grown on minimal NCE media supplemented with Cby, DMB, and EA-P. Corrinoid standards, gray; Cby eluted at 12 min, Cbi eluted at 17.2 min, and Cbl eluted at 28.8 min. Extracted corrinoid from l-Thr-P-supplemented cell cultures (bottom black trace) eluted at 12 min (Cby) and 29.8 min (UNK1, cobamide of unknown identity 1). Extracted corrinoids from cell cultures grown with EA-P (upper black trace) eluted at 21.6 (UNK2, cobamide of unknown identity 2) and 28.3 (norCbl) min. The chemical structure of norcobalamin (peak at 28.3 min) is shown. f.m., formula mass.