TABLE 1.
Strains and plasmidsa
| Strain or plasmid | Relevant genotype | Reference/source |
|---|---|---|
| S. enterica serovar Typhimurium strain LT2 derivatives | ||
| TR6583 (formerly SA2929) | metE205 ara-9 | K. Sanderson via J. Roth |
| JE9534 | ΔmetE ara-9 cbiB1344::cat+ | |
| JE9535 | ΔmetE ara-9 ΔcbiB1345c | |
| Derivatives of TR6583 | ||
| JE2216 | cobD1302::Tn10d(cat+)b | Laboratory collection |
| JE4107 | zeb6400::Tn10d(cat+)b | Laboratory collection |
| JE6045 | cobD1302::Tn10d(cat+)/pT7-7b | Laboratory collection |
| JE8126 | cbiB1308::cat+ | |
| JE8185 | ΔcbiB1309 | |
| JE8214 | cobS1312::cat+ | |
| JE8268 | ΔcobU1315 ΔycfN112 | Laboratory collection |
| JE8311 | ΔcobU1315 ΔycfN112/pSU39 | Laboratory collection |
| JE8312 | ΔcobU1315 ΔycfN112/pCOBY38 | Laboratory collection |
| JE8836 | cobD1302::Tn10d(cat+)/pCBIB4b | |
| JE9266 | zeb64500::Tn10d(cat+) cbiB1260/pT7-7 | |
| JE9268 | zeb64500::Tn10d(cat+) cbiB1261/pT7-7 | |
| JE9283 | zeb64500::Tn10d(cat+) cbiB1265/pT7-7 | |
| JE9290 | ΔcbiB1309/pCBIB4 | |
| JE9291 | ΔcbiB1309/pT7-7 | |
| JE9515 | cbiB1308::cat+ ΔcobU1315 ΔycfN112 | |
| JE9709 | JE9515/pCBIB4/pCOBY38 | |
| JE9710 | JE9515/pCBIB4/pSU39 | |
| JE9713 | JE9515/pCBIB34/pCOBY38 | |
| JE9714 | JE9515/pCBIB34/pSU39 | |
| JE9715 | JE9515/pCBIB36/pCOBY38 | |
| JE9716 | JE9515/pCBIB36/pSU39 | |
| JE9717 | JE9515/pCBIB35/pCOBY38 | |
| JE9718 | JE9515/pCBIB35/pSU39 | |
| JE9719 | JE9515/pCBIB37/pCOBY38 | |
| JE9720 | JE9515/pCBIB37/pSU39 | |
| JE9721 | JE9515/pCBIB39/pCOBY38 | |
| JE9722 | JE9515/pCBIB39/pSU39 | |
| JE9723 | JE9515/pCBIB38/pCOBY38 | |
| JE9724 | JE9515/pCBIB38/pSU39 | |
| JE9725 | JE9515/pCBIB40/pCOBY38 | |
| JE9726 | JE9515/pCBIB40/pSU39 | |
| E. coli strains | ||
| CC118 | araD139 Δ(ara leu)7697 ΔlacX74 phoAΔ20 galE galK thi rpsE rpoB argE(Am) recA1 | 22 |
| XL10Gold | Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F′ proAB lacIZDM15 Tn10 (Tetr) Amy Cmr] | Stratagene |
| DH5α | φ80Δlac(lacZ)M15 recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Laboratory collection |
| JE7306 | DH5α/pCBIB13 | |
| JE7307 | XL10Gold/pCBIB18 | |
| JE7308 | DH5α/pCBIB15 | |
| JE9437 | XL10Gold/pCBIB12 | |
| JE9438 | XL10Gold/pCBIB16 | |
| JE9439 | XL10Gold/pCBIB14 | |
| JE9790 | DH5α/pCBIB17 | |
| JE9791 | DH5α/pCBIB19 | |
| JE9792 | DH5α/pCBIB20 | |
| Derivatives of CC118 | ||
| JE9033 | CC118/pCBIB33 (loop VI, phoA) | |
| JE9034 | CC118/pCBIB28 (region IV, phoA) | |
| JE9035 | CC118/pCBIB32 (loop V-VI, phoA) | |
| JE9538 | CC118/pCBIB21 (region I, lacZ) | |
| JE9638 | CC118/pCBIB29 (region IV, lacZ) | |
| JE9639 | CC118/pCBIB31 (loop IV-V, lacZ) | |
| JE9641 | CC118/pCBIB25 (region III, phoA) | |
| JE9775 | CC118/pCBIB22 (region II, phoA) | |
| JE9776 | CC118/pCBIB24 (region II, lacZ) | |
| JE9777 | CC118/pCBIB26 (region III, lacZ) | |
| S. enterica serovar Typhimurium strains | ||
| JE9778 | CC118/pCBIB30 (loop IV-V, phoA) | |
| JE9779 | CC118/pCBIB4 | |
| Plasmids | ||
| pSU39 | Cloning vector; kan+ | |
| pT7-7 | Cloning vector; bla+ | 34 |
| pCOBY38 | cobY+ (M. mazei) in pSU39 Kan+ | Laboratory collection |
| pCBIB4 | cbiB+ in pT7-7 bla+ | Laboratory collection |
| pCH40 | bla::phoA | 17 |
| pSKS107 | lacZYA+bla+ | 8 |
| Derivatives of pT7-7 | ||
| pCBIB12 | cbiB1346 (PstI site at cbiB nucleotide no. 70) | |
| pCBIB13 | cbiB1347 (PstI site at cbiB nucleotide no. 106) | |
| pCBIB14 | cbiB1348 (PstI site at cbiB nucleotide no.169) | |
| pCBIB15 | cbiB1349 (PstI site at cbiB nucleotide no. 229) | |
| pCBIB17 | cbiB1351 (PstI site at cbiB nucleotide no. 403) | |
| pCBIB18 | cbiB1352 (PstI site at cbiB nucleotide no. 574) | |
| pCBIB19 | cbiB1353 (PstI site at cbiB nucleotide no. 778) | |
| pCBIB20 | cbiB1354 (PstI site at cbiB nucleotide no. 940) | |
| pCBIB21 | cbiB-lacZ (last CbiB aa P23) | |
| pCBIB22 | cbiB-phoA (last CbiB aa F35) | |
| pCBIB24 | cbiB-lacZ (last CbiB aa F35) | |
| pCBIB25 | cbiB-phoA (last CbiB aa V56) | |
| pCBIB26 | cbiB-lacZ (last CbiB aa A76) | |
| pCBIB28 | cbiB-phoA (last CbiB aa Q136) | |
| pCBIB29 | cbiB-lacZ (last CbiB aa Q136) | |
| pCBIB30 | cbiB-phoA (last CbiB aa Y191) | |
| pCBIB31 | cbiB-lacZ (last CbiB aa Y191) | |
| pCBIB32 | cbiB-phoA (last CbiB aa G259) | |
| pCBIB33 | cbiB-phoA (last CbiB aa C313) | |
| pCBIB34 | cbiB1355 (encodes CbiBH24A) | |
| pCBIB35 | cbiB1356 (encodes CbiBR121A) | |
| pCBIB36 | cbiB1357 (encodes CbiBE150A) | |
| pCBIB37 | cbiB1358 (encodes CbiBT179I) | |
| pCBIB38 | cbiB1359 (encodes CbiBD181N) | |
| pCBIB39 | cbiB1360 (encodes CbiBG195D) | |
| pCBIB40 | cbiB1361 (encodes CbiBL262A) |
a
Strains and plasmids were constructed during the course of this work unless stated otherwise. aa, amino acid.
b
Strain is described in reference 13.
c
The cbiB1345 gene encodes CbiBW126Z Δ127-319.