TABLE 2.

PstI restriction sites and primers^a

Plasmid	PstI site location (nucleotide no. from the start of cbiB)	Primers
pCBIB12	70	5′-GGCCCCTGCAGGTACGCTGGATAGG-3′
		5′-CAGCGTACCTGCAGGGGCCAGTGTTG-3′
pCBIB13	106	5′-TTTAATTACGTTTCTGCAGCGTATTGTGCG-3′
		5′-CGCACAATACGCTGCAGAAACGTAATTAAA-3′
pCBIB14	169	5′-GGCGTACTGCAGGTTGTGGTTGTCG-3′
		5′-CCACAACCTGCAGTACGCCGCCGCC-3′
pCBIB15	229	5′-TACTGGCGCTGGCGCTGCAGATTCACCCCTGGTT-3′
		5′-AACCAGGGGTGAATCTGCAGCGCCAGCGCCAGTA-3′
pCBIB17	403	5′-GATACGTCGCAACTGCAGCCAGCGCAGATC-3′
		5′-GATCTGCGCTGGCTGCAGTTGCGACGTATC-3′
pCBIB18	574	5′-CAAACATGAAAAATACCTGCAGATTGGTATGGTCAGCG-3′
		5′-CGCTGACCATACCAATCTGCAGGTATTTTTCATGTTTG-3′
pCBIB19	778	5′-GCCGGCGCCTTAGGACTGCAGCTCGGTGGCCCA-3′
		5′-TGGGCCACCGAGCTGCAGTCCTAAGGCGCCGGC-3′
pCBIB20	940	5′-TGCGGCGCGGTGCCTGCAGTCTGGCGTGG-3′
		5′-GGCCACGCCAGACTGCAGGCACCGCGCCG-3′

^aPstI restriction sites are shown in bold type.