TABLE 4.
Activity of CbiB-PhoA and CbiB-LacZ fusion proteinsa
| Residue no. of CbiB fusion site | Plasmid encoding CbiB-PhoA fusion | Plasmid encoding CbiB-LacZ fusion | No. of molecules ± SD of alkaline phosphatase activity | No. of molecules ± SD of β-galactosidase activity |
|---|---|---|---|---|
| Control | pCBIB4 (no fusion) | pCBIB4 (no fusion) | 2 ± 1 | 0.2 ± 0.1 |
| P23 | None | pCBIB21 | ND | NA |
| F35 | pCBIB22 | pCBIB24 | 8 ± 1 | 0.3 ± 0.1 |
| V56 | pCBIB25 | None | 3 ± 0.3 | ND |
| A76 | None | pCBIB26 | ND | 8 ± 2.4 |
| Q136 | pCBIB28 | pCBIB29 | 1 ± 0.2 | 0.3 ± 0.02 |
| Y191 | pCBIB30 | pCBIB31 | 84 ± 6 | 2 ± 1 |
| G259 | pCBIB32 | None | 2 ± 1 | ND |
| C313 | pCBIB33 | None | 79 ± 6 | ND |
a
Units are expressed as the number of molecules ± standard deviation (SD) of active alkaline phosphatase or active β-galactosidase per cell, respectively. ND, not determined; NA, no activity. Data obtained from control experiments were considered background noise.