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Growth behavior of strains synthesizing CbiB variantsa
| pCbiB variant | Doubling time (h) |
|---|---|
| pCbiBH24A | 1.6 |
| pCbiBE150A | 23 |
| pCbiBR121A | 2.0 |
| pCbiBT179I | 1.9 |
| pCbiBG195D | 7.1b |
| pCbiBD181N | 23.5 |
| pCbiBL262A | 2.2 |
| pCbiBWT | 1.8 |
Cultures were grown in NCE medium supplemented with glycerol, Mg2+, Cby, and DMB, as indicated in Materials and Methods.
Time period includes a 6-h lag.
