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Streptavidin affinity purification of Sulfo-NHS-SS-Biotin-labeled A. phagocytophilum surface proteins. In lane 1, Sulfo-NHS-SS-Biotin-labeled A. phagocytophilum surface proteins were separated by 10% SDS-PAGE and stained with GelCode blue. Bands 1 to 7 were subjected to Nano-LC/MS/MS analysis. The marker lane contained Precision Plus prestained protein standards (Bio-Rad).
