TABLE 1.

Surface-exposed proteins of A. phagocytophilum analyzed by Nano-LC/MS/MS^a

Band	Annotation or gene locus tag	Predicted molecular mass (Da)	Nano-LC/ MS/MS sequence coverage by amino acids (%)	Accession no.	Predicted protein function	Pfam family(ies)	Presence of signal peptide sequence (position)
1	Hypothetical protein APH_0441	65,911	4	YP_505044	Unknown; putative lipoprotein	NAb	Yes (35)c
2	Outer membrane protein, Omp85 family	85,709	30	YP_505741	Cell envelope biogenesis	PF07244, PF01103	Yes (22)c,d
	Translation elongation factor G	76,234	32	YP_505591	GTPase, power translation	PF00009, PF03144, PF03764, PF00679	No
	Chaperone protein DnaK	70,019	16	YP_504953	Chaperone	PF00012	No
	ppiC/parvulin rotamase family protein	67,535	15	YP_505198	Accelerate the folding of proteins	NA	No
3	Hypothetical protein APH_0404 (Asp62)	63,987	42	YP_505009	Unknown	NA	Yes (19)c
	60-kDa chaperonin (GroEL)	57,282	35	YP_504857	Chaperone	PF00118	No
	Pentapeptide repeat family protein	61,870	5	YP_505709	Uncharacterized secreted protein (YP_198411) containing pentapeptide repeats (Wolbachia endosymbiont strain TRS from B. malayi)	PF00805	No
4	Hypothetical protein APH_0405 (Asp55)	57,595	13	YP_505010	Unknown	NA	Yes (22)d
5	P44-18ES, P44 outer membrane protein expression locus with P44-18	45,827	38	YP_505752	Porin	PF01617	Yes (20)c,d
	P44-2a and P44-2b	38,478 and 44,912	39	YP_505715 and YP_505759	P44 paralog	PF01617
	P44-59	NA	10	AAQ16676	P44 paralog	NA
7	Major outer membrane protein Omp-1A	31,845	41	YP_505858	Unknown	PF01617	Yes (19)d
	Type IV secretion protein VirB8-1	27,582	16	YP_505898	Transportation of macromolecules across bacterial inner and outer membranes	PF04335	No
	Thiol:disulfide oxidoreductase	27,842	8	YP_504749	Protein folding and stabilization	PF01323	Yes (21)d
	Cochaperone GrpE	22,761	8	YP_504670	Chaperone	PF01025	No

^aThe program SignalP 3.0 (http://www.cbs.dtu.dk/services/SignalP) was used to predict the presence of N-terminal signal peptides (5). The putative lipoprotein was predicted by LipoP 1.0.

^bNA, not available.

^cPredicted by SignalP-NN.

^dPredicted by SignalP-HMM.