FIG. 1.

LGL enzyme activity in S. mutans soluble cell extracts subjected to various environmental factors. Initial rates were measured in triplicate and monitored by the increase in absorbance at 240 nm due to the conversion of hemithioacetal to S-d-lactoylglutathione. (A) UA159 (▪) and LGLKO (□); (B) S. mutans UA159 cells grown to mid-log phase at pH 7.5 (○) and pH 5.0 (•); (C) S. mutans UA159 acid-adapted cells (•) and nonadapted cells (○); (D) S. mutans UA159 cells grown to mid-logarithmic growth phase (○) and stationary growth phase (•); (E) S. mutans UA159 cells at pH 7.0 subjected to glucose starvation (○) and 200 mM glucose (•); (F) S. mutans UA159 cells at pH 5.0 subjected to glucose starvation (○) and 200 mM glucose (•).