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. 2007 Aug 24;189(21):7531–7538. doi: 10.1128/JB.00767-07

FIG. 1.

FIG. 1.

Confirmation of Δetx::catP mutants. (A and B) PCR analysis using etx- and catP-specific primers, respectively. Lanes 2 to 6 contained strains CN1020, JIR4891, CN3718, and JIR4892 and recombination plasmid pJIR3077, respectively. Lanes 1 and 7 contained size markers (Promega PCR marker and HindIII-digested λcI857, respectively). (C and D) Southern blots of HindIII-digested genomic DNA obtained using etx- and catP-specific probes, respectively. The contents of lanes 2 to 6 were the same as those described above. Lane 1 contained HindIII-digested λcI857 molecular size markers. (E) Representation of the etx region (based on previous studies [30]) in the wild-type ɛ-toxin plasmids pJIR3118 and pJIR3119, compared to the same region after insertional inactivation of the etx gene by allelic exchange with the recombination vector pJIR3077. The positions of HindIII sites (H) are indicated, as are the sizes of the predicted HindIII fragments (in kb). The etx and catP probes used for Southern blotting are indicated by the solid lines under the genes.