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. 2007 Aug 24;189(21):7531–7538. doi: 10.1128/JB.00767-07

FIG. 4.

FIG. 4.

ɛ-Toxin-mediated cytopathic effects of culture supernatants. Monolayers of MDCK cells were incubated for 24 h in the presence of trypsin-treated culture supernatants from different strains before examination with an Olympus 1X71 inverted microscope. ImageJ 1.37v software (http://rsb.info.nih.gov/ij/index.html) was used to view the images. (A) JIR325. (B) CN1020 Δetx::catP. (C) Medium alone. (D) CN1020. (E) CN1020 pretreated with MAb 5B7. (F) CN1020 pretreated with ErmB-specific antibody. (G) JIR12012 [JIR325(pJIR3118)]. (H) JIR12012 pretreated with MAb 5B7. (I) JIR12012 pretreated with ErmB-specific antibody. (J) JIR4983 [JIR325(pJIR3118Δetx::catP)]. (K) JIR4983 pretreated with MAb 5B7. (L) JIR4983 pretreated with ErmB-specific antibody. The culture supernatant from CN1020 was diluted 50-fold prior to use. Bar, 100 μm.