TABLE 5.
Effects of ΔnarL, ΔnarP, ΔnsrR and Δfnr null alleles on expression from a Φ(nnrS-lacZ) fusion during anaerobic growth in defined medium
| Strain | Genotype
|
LacZ sp act (Miller units)a
|
Activation by:
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| narL | narP | nsrR | fnr | No addition | With NO3− | With NO2− | NO3− | NO2− | |
| VJS9438 | + | + | + | + | 43 | 1,000 | 94 | 23 | 2.2 |
| VJS9546 | − | + | + | + | 46 | 95 | 82 | 2.1 | 1.8 |
| VJS9547 | + | − | + | + | 52 | 990 | 91 | 19 | 1.8 |
| VJS9548 | + | + | − | + | 63 | 900 | 86 | 14 | 1.4 |
| VJS9545 | + | + | + | − | 60 | 1,100 | 300 | 18 | 5.0 |
a
Strains were cultured to the early exponential phase in glucose defined medium.