TABLE 6.
Effects of ΔnarL, ΔnarP, ΔnsrR, and Δfnr null alleles on expression from a Φ(nnrS-lacZ) fusion during anaerobic growth in complex medium
| Strain | Genotype
|
LacZ sp act (Miller units)a
|
Activation by:
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| narL | narP | nsrR | fnr | No addition | With NO3− | With SNP | NO3− | SNP | |
| VJS9438 | + | + | + | + | 54 | 870 | 54 | 16 | 2.0 |
| VJS9546 | − | + | + | + | 54 | 85 | 54 | 1.6 | 1.3 |
| VJS9547 | + | − | + | + | 64 | 610 | 64 | 10 | 1.9 |
| VJS9548 | + | + | − | + | 71 | 630 | 71 | 9 | 1.4 |
| VJS9545 | + | + | + | − | 130 | 1,000 | 130 | 8 | 3.9 |
a
Strains were cultured to the early exponential phase in enriched medium with glucose.