TABLE 3.

Intracellular proteins significantly altered in abundance in the pigX mutant compared with their levels in Serratia strain ATCC 39006

Relative level of pigX mutant	Spot no.a	Protein identity	Nameb	Change in abundance (fold)	Bacteriumb	Accession no.b
Increased	1	Glycerol kinase	GlpK	1.10	Escherichia coli	gi 1134966
	2	Aldehyde dehydrogenase	PutA	1.31	Shewanella baltica OS155	gi 68541744
	3	GTP cyclohydrolase I	Sden_1513	1.28	Shewanella denitrificans OS217	gi 91792870
	4	l-Prolyl-PCP dehydrogenase	PigA	2.22	Serratia strain ATCC 39006	gi 55581716
	5	Phosphoserine aminotransferase	SerC	1.17	Yersinia enterocolitica	gi 134441
	6	ABC-type branched-chain amino acid transport systems, periplasmic component	LivK	1.27	Yersinia mollaretii ATCC 43969	gi 77961773
	7	Acyl-carrier protein, enoyl reductase (NADH)	FabI	1.41	Erwinia carotovora subsp. atroseptica	gi 49611426
	8	Aminopeptidase N	PepN	1.69	Rubrivivax gelatinosus PM1	gi 47573617
	9	Predicted periplasmic lipoprotein involved in iron transport	YP_1538	1.13	Yersinia pestis biovar microtus strain 91001	gi 45436217
	10	HBC O-methyl transferase	PigF	2.07	Serratia strain ATCC 39006	gi 55581721
		Periplasmic glucan biosynthesis proteinc	OpgG	1.78	Pseudomonas syringae pv. tomato strain DC3000	gi 28855528
Decreased	11 and 12	Aspartate ammonia-lyase 2	AspA	−1.34 −1.25	Erwinia carotovora subsp. atroseptica	gi 49610097
	13	Glycerol dehydrogenase	GldA	−1.26	Erwinia carotovora subsp. atroseptica	gi 49610315

^aThe spot number is the spot number in the 2D-DiGE image in Fig. 3.

^bProtein name, bacterium name, and accession number refer to the top hits for the Serratia strain ATCC 39006 peptides identified by MS using MASCOT.

^cThe spot described here was picked from the original “difference in gel analysis” 2D-DiGE analysis (not shown) and identified by MS.