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. 2007 Aug 22;81(21):11790–11797. doi: 10.1128/JVI.01113-07

FIG. 3.

FIG. 3.

VP1/2cbd is associated with capsids isolated from PRV-infected cell nuclei. (A) Representative image of capsids isolated from the infected cell nuclei. (B) Fluorescence imaging of capsids isolated from PK15 cells stably expressing GFP-VP1/2cbd infected with wild-type RFP-capsid virus (left panels), ΔUL25 RFP-capsid virus (center panels), or PK15 cells infected with an RFP-capsid/GFP-VP22 virus (right panels). Arrowheads indicate capsids emitting detectable GFP fluorescence. (C) Percentage of RFP-capsid fusions emitting detectable GFP fluorescence, as shown in panel B. Error bars are standard deviations. (D) The presence of VP5 and GFP fusion proteins was detected in each capsid preparation by Western blot analysis. Predicted molecular sizes of proteins are indicated at right, which were consistent with molecular size markers. α, anti.