FIG. 4.
MV-N lacking eIF3-p40-binding ability shows no inhibitory effect on translation. Increasing amounts of recombinant GST or GST-NΔ2c were incubated with rabbit reticulocyte lysates. A reporter RNA encoding the luciferase gene was added and translated in the presence of [35S]methionine. Translated products were separated by SDS-PAGE and detected by autoradiography. The amount of luciferase synthesized was quantified by densitometric analysis. The results shown represent means of three experiments.